Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:500Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR8 antibody [N1], N-term diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Product Name | mGluR8 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Metabotropic Glutamate Receptor 8 (glutamate receptor, metabotropic 8)L-glutamate is the major excitatory neurotransmitter in the central nervous system and activates both ionotropic and metabotropic glutamate receptors. Glutamatergic neurotransmission is involved in most aspects of normal brain function and can be perturbed in many neuropathologic conditions. The metabotropic glutamate receptors are a family of G protein-coupled receptors, that have been divided into 3 groups on the basis of sequence homology, putative signal transduction mechanisms, and pharmacologic properties. Group I includes GRM1 and GRM5 and these receptors have been shown to activate phospholipase C. Group II includes GRM2 and GRM3 while Group III includes GRM4, GRM6, GRM7 and GRM8. Group II and III receptors are linked to the inhibition of the cyclic AMP cascade but differ in their agonist selectivities. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Metabotropic Glutamate Receptor 8. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouseRat |
| Predicted Reactivity | Rhesus Monkey, BovineRhesus Monkey (100%), Bovine (100%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | FLJ41058 antibody GLUR8 antibody GPRC1H antibody MGC126724 antibody MGLUR8 antibody mGlu8 antibody GRM8 antibody metabotropic glutamate receptor 8 antibody "glutamate receptor metabotropic 8 antibody" |
|---|---|
| Molecular Weight(MW) | 102 kDa (note) (2) |
| Cellular Localization | Cell membrane; Multi-pass membrane protein |
Application
Sample (30 ug of whole cell lysate) A: A549 7.5% SDS PAGE diluted at 1:500
Application
Various tissue extracts (50 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with mGluR8 antibody [N1], N-term diluted at 1:500. The signal was developed with Trident ECL plus-Enhanced.| Positive Control | A549 , mouse brain , rat brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
