Application
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 ug/ml.Application
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 ug/ml.| Product Name | Mimitin antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MimitinMimitin, a small mitochondrial protein, whose transcription is directly stimulated by c-Myc, is highly expressed in 80% of esophageal squamous cell carcinomas (ESCC). Suppression of Mimitin expression by RNA interference had no effect in cancerous cell lines such as human cervical carcinoma or hepatocarcinoma cell lines, but caused a decrease in cell proliferation in human glioblastoma, embryonic lung fibroblastic cells, and ESCC, suggesting Mimitin may play a special role in these types of cells. Mimitin expression is also regulated by MAPK kinases and IL-1, but not through the NF-kB-related pathway. It will interact with the microtubular protein MAP1S and can affect the activities of caspase-3 and -7 in cells stimulated to develop apoptosis. Other experiments suggest that Mimitin also acts as a molecular chaperone for the assembly of the mitochondrial complex I. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAICC/IFWB |
| ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | assembly factor2 NDUFAF2 MMTN NADH dehydroxygenase (ubiquinone) 1 alpha subcomplex assembly factor 2 Mimitin NDUFA12L |
|---|---|
| Target | Mimitin |
| Tissue Specificity | This antibody is specific for Mimitin Internal |
Application
Western blot analysis of Mimitin in Raji cell lysate with Mimitin antibody at (A) 1 and (B) 2 ug/ml.
Application
Immunofluorescence of Mimitin in Raji cells with Mimitin antibody at 20 ug/ml.| Positive Control | Blocking Peptide: Cat.No. GTX85237-PEP - Mimitin Peptide Lysates: Cat. No. GTX27908 - Raji Cell Lysate |
|---|---|
| Application Notes | ICC/IF:1:200~1:500 WB:1:500~1:2000 Notes:Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | PBS containing 0.02% sodium azide |
