Application
Human Lung stained with anti-MIP-1 alpha antibody| Product Name | MIP1 alpha antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MIP1 alphaMacrophage Inflammatory Protein 1 alpha (MIP1 alpha) and MIP1 beta, two closely related but distinct proteins, were originally co-purified from medium conditioned by a LPS-stimulated murine macrophage cell line. Mature mouse MIP1 alpha shares approximately 77% and 70% amino acid identity with human MIP1 alpha and mouse MIP1 beta, respectively. MIP1 proteins are expressed primarily in T cells, B cells, and monocytes after antigen or mitogen stimulation. Has adhesive effects on lymphocytes. MIP1 alpha can inhibit the proliferation of hematopoietic stem cells in vitro as well as in vivo. A signal transducing receptor, designated the C-C chemokine receptor-1 (C-C CKR-1) with seven transmembrane domains that bind MIP1 alpha, MIP1 beta, MCP1 and RANTES with varying affinities, has been isolated. |
| Immunogen | Synthetic peptide: EEW VQKYVSDLEL SA , corresponding to C terminal amino acids 77-92 of Human Macrophage Inflammatory Protein 1 alpha / CCL3 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-P |
| Recommended dilutions: IHC-P: 1/50 for 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | G0S19-1 LD78ALPHA MIP-1-alpha MIP1A SCYA3 MIP 1 alpha MIP1alpha |
|---|---|
| Target | MIP1 alpha |
| Cellular Localization | Secreted |
Application
Human Lung stained with anti-MIP-1 alpha antibody| Positive Control | Lung carcinoma |
|---|---|
| Application Notes | Recommended dilutions: IHC-P: 1/50 for 30 minutes at room temperature. Perform heat mediated antigen retrieval before commencing with IHC staining protocol. Not yet tested in other applications. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 1% BSA and 0.09% sodium azide |
