Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207) Antibody .Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using MKK6 (Phospho-Ser207) Antibody .Application
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207) Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207)| Product Name | MKK6 (phospho Ser207) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MKK6 (Phospho Ser207)Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in MAP kinase p38 exclusively. |
| Immunogen | Peptide sequence around phosphorylation site of serine 207 (V-D-S(p)-V-A) derived from Human MKK6. |
| Modification | p-Ser207 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK6 MEK6 MKK6 PRKMK6 SAPKK3 MAP2K6 SAPK kinase 3 dual specificity mitogen-activated protein kinase kinase 6 MAPK/ERK kinase 6 MEK 6 MAPKK 6 protein kinase mitogen-activated kinase 6 (MAP kinase kinase 6) stress-activated protein kinase kin |
|---|---|
| Target | MKK6 (Phospho Ser207) |
| Molecular Weight(MW) | 41 (note) |
Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6 (Phospho-Ser207) Antibody .
Application
Immunofluorescence staining of methanol-fixed Hela cells showing cytoplasmic staining using MKK6 (Phospho-Ser207) Antibody .
Application
Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using MKK6 (Phospho-Ser207) Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from HL60 cells untreated or treated with PMA using MKK6(Phospho-Ser207)| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
