Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK6 Antibody .Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK6 Antibody (left) or the same antibody preincubated with blocking peptide (right).Application
Western blot analysis of extracts from MDA, 293 and Hela cells using MKK6 Antibody .Application
Western blot analysis of extracts from MDA, 293 and Hela cells using MKK6(Ab-207) Antibody| Product Name | MKK6 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MKK6MEK6 is a member of MAPKK protein kinase family. By using degenerate oligonucleotide primers from the conserved kinase domains of MKK3 and MKK4 two human cDNAs and 1 murine cDNA encoding closely related proteins of the MKK family were cloned. The two human clones appear to be different isoforms of the same gene generated by differential splicing: the shorter clone was designated MKK6, encodes a 278-amino acid protein, while the longer clone, designated MKK6b, encodes a 334-amino acid protein. MKK6 is about 80% identical to MKK3 and 40% identical to MKK4. 1.7-kb human MKK6 transcript is highly expressed in skeletal muscle, while an MKK6b-specific probe detected mRNA bands of 1.8, 2.4, and 4.5 kb that are enriched in heart, skeletal muscle, pancreas and liver. MKK6 plays an important role in intracellular signaling pathways leading toward activation of the p38 MAP kinase . MEK6 phosphorylates and activates p38 in response to inflammatory cytokines or environmental stress. As an essential component of p38 MAPK mediated signal transduction pathway, this gene is involved in many cellular processes such as stress induced cell cycle arrest, transcription activation and apoptosis. |
| Immunogen | Peptide sequence around aa.205~209 (V-D-S-V-A) derived from Human MKK6. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MAPKK6 MEK6 MKK6 PRKMK6 SAPKK3 MAP2K6 SAPK kinase 3 dual specificity mitogen-activated protein kinase kinase 6 MAPK/ERK kinase 6 MEK 6 MAPKK 6 protein kinase mitogen-activated kinase 6 (MAP kinase kinase 6) stress-activated protein kinase kin |
|---|---|
| Target | MKK6 |
| Molecular Weight(MW) | 41 (note) |
Application
Immunofluorescence staining of methanol-fixed Hela cells using MKK6 Antibody .
Application
Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MKK6 Antibody (left) or the same antibody preincubated with blocking peptide (right).
Application
Western blot analysis of extracts from MDA, 293 and Hela cells using MKK6 Antibody .
Application
Western blot analysis of extracts from MDA, 293 and Hela cells using MKK6(Ab-207) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000, IHC-P: 1:50~1:100, ICC/IF: 1:100~1:200. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
