Rabbit monoclonal antibody [EP558Y] to MKK6 (C-term)Mitogen-acitvated protein kinase kinase 6 (MKK6) belongs to the serine/threonine protein kinase family and the MAPK kinase subfamily (MAP2K, MKK or MEKs). MAPKs require dual phosphorylation on threonine and tyrosine residues, an activation step carried out by MAPK kinase . MKK6, closely related to MKK3, efficiently phosphorylates and activates p38 MAP kinase in response to inflammatory cytokines and environmental stress but not JNK or ERK . MTK1 phosphorylates MKK6 on serine 207 and threonine 211 .
Immunogen
A synthetic peptide corresponding to residues in the C-term of human MKK6 was used as immunogen.
Key Feature
Clonality
Monoclonal
Isotype
IgG
Clone Number
EP558Y
Host Species
Rabbit
Tested Applications
IHC-PWB
For WB: Use at a dilution of 1:10,000:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Target Information
Gene Synonyms
MEK6 MKK6 PRKMK6 MAP2K6 MAPKK6 SAPKK3
Target
MKK6 (C-term)
Molecular Weight(MW)
36 (note)
Application
Application
B. Immunohistochemical analysis of paraffin-embedded human heart muscle using anti-MKK6 RabMAb (cat # ).
Application
A. Western blot analysis on Hela cell lysate using anti-MKK6 RabMAb (cat. # ) dilution 1:10000.
Application Notes
For WB: Use at a dilution of 1:10,000:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 °C. Stable for 12 months from date of receipt.
Storage Buffer
50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA.