Application
Western blot analysis of extracts of various cell lines, using MLH1 antibody.Application
Immunofluorescence analysis of U2OS cell using MLH1 antibody. Blue: DAPI for nuclear staining.| Product Name | MLH antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MLHThis gene was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). It is a human homolog of the E. coli DNA mismatch repair gene mutL, consistent with the characteristic alterations in microsatellite sequences (RER+phenotype) found in HNPCC. Alternative splicing results in multiple transcript variants encoding distinct isoforms. Additional transcript variants have been described, but their full-length natures have not been determined.[provided by RefSeq, Nov 2009] |
| Immunogen | Recombinant protein of human MLH1 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| WB: 1:500 - 1:2000, IF: 1:50 - 1:100: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | hMLH1 HNPCC2 MLH1 mutL homolog 1 HNPCC FCC2 COCA2 |
|---|---|
| Target | MLH |
Application
Western blot analysis of extracts of various cell lines, using MLH1 antibody.
Application
Immunofluorescence analysis of U2OS cell using MLH1 antibody. Blue: DAPI for nuclear staining.| Application Notes | WB: 1:500 - 1:2000, IF: 1:50 - 1:100: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
