Rabbit polyclonal antibody to MlxTranscription regulator. Forms a sequence-specific DNA-binding protein complex with MAD1, MAD4, MNT, WBSCR14 and MLXIP which recognizes the core sequence 5'-CACGTG-3'. The TCFL4-MAD1, TCFL4-MAD4, TCFL4-WBSCR14 complexes are transcriptional repressors. Plays a role in transcriptional activation of glycolytic target genes. Involved in glucose-responsive gene regulation.
Immunogen
The antiserum was produced against synthesized peptide derived from human Mlx.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
HumanMouseRat
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Mlx Max-like bHLHZip protein BigMax protein protein Mlx MAD7 transcription factorlike protein4 Max-like protein X Maxlike protein X TCFL4 transcription factorlike4 transcription factor-like 4 transc MXD7 transcription factorlike protein 4 Max
Target
Mlx
Molecular Weight(MW)
33 kDa (note)
Tissue Specificity
Mlx Antibody detects endogenous levels of total Mlx protein.
Cellular Localization
Found predominantly in the cytoplasm. Found predominantly in the cytoplasm. Found predominantly in the nucleus.
Application
Application
Western blot analysis of extracts from LOVO cells, using Mlx Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.