Application
Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.Application
Western blot analysis of MLX Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.Application
Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.| Product Name | MLX antibody, Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MLX (Internal)The product of this gene belongs to the family of basic helix-loop-helix leucine zipper (bHLH-Zip) transcription factors. These factors form heterodimers with Mad proteins and play a role in proliferation, determination and differentiation. This gene product may act to diversify Mad family function by its restricted association with a subset of the Mad family of transcriptional repressors, namely, Mad1 and Mad4. Alternatively spliced transcript variants encoding different isoforms have been identified for this gene. [provided by RefSeq] |
| Immunogen | KLH conjugated synthetic peptide between 131~160 amino acids from the Center region of human MLX. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MLX Max-like bHLHZip protein BigMax protein Transcription factorlike protein4 Max-like protein X Maxlike protein X TCFL4 Protein Mlx Transcription factor-like protein 4 Maxlike bHLHZip protein Transcription factorlike protein 4 Max like protein |
|---|---|
| Target | MLX |
| Molecular Weight(MW) | 33.3 kDa (note) |
| Cellular Localization | Found predominantly in the cytoplasm. Found predominantly in the cytoplasm. Found predominantly in the nucleus. |
Application
Flow cytometric analysis of NCI-H292 cells using MLX Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
Formalin-fixed and paraffin-embedded human hepatocarcinoma with MLX Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Application
Western blot analysis of MLX Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). MLX (arrow) was detected using the purified Pab.
Application
Western blot analysis of MLX (arrow) using rabbit polyclonal MLX Antibody (Center) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MLX gene.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
