Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC antibody at 1:500 dilution.Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC antibody at 1:100 dilution.Application
Sample(30 ug whole cell lysate) A:293T B:Raji 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMACHC antibody at 1:500 dilution.| Product Name | MMACHC antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MMACHC (methylmalonic aciduria (cobalamin deficiency) cblC type, with homocystinuria)The exact function of the protein encoded by this gene is not known, however, its C-terminal region shows similarity to TonB, a bacterial protein involved in energy transduction for cobalamin (vitamin B12) uptake. Hence, it is postulated that this protein may have a role in the binding and intracellular trafficking of cobalamin. Mutations in this gene are associated with methylmalonic aciduria and homocystinuria type cblC. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MMACHC. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Pig, Rhesus Monkey, BovineRat (85%), Pig (91%), Rhesus Monkey (96%), Bovine (90%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | DKFZp564I122 antibody FLJ25671 antibody RP11-291L19.3 antibody cblC antibody MMACHC antibody methylmalonic aciduria and homocystinuria type C protein antibody "methylmalonic aciduria (cobalamin deficiency) cblC type with homocystinuria antibody" |
|---|---|
| Molecular Weight(MW) | 32 kDa (note) |
Application
Immunohistochemical analysis of paraffin-embedded C2C12 xenograft, using MMACHC antibody at 1:500 dilution.
Application
Sample (50 ug of whole cell lysate) A: Mouse brain 12% SDS PAGE diluted at 1:1000
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MMACHC antibody at 1:100 dilution.
Application
Sample(30 ug whole cell lysate) A:293T B:Raji 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human hepatoma, using MMACHC antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
