Application
MMP12 antibody [N3C1], Internal detects MMP12 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500.Application
Immunofluorescence analysis of methanol-fixed H1299, using MMP12 antibody at 1:500 dilution.Application
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using MMP12 antibody at 1:500 dilution.Application
Sample(30 µg whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Product Name | MMP12 antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MMP12 (matrix metallopeptidase 12 (macrophage elastase))Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. It is thought that the protein encoded by this gene is cleaved at both ends to yield the active enzyme, but this processing has not been fully described. The enzyme degrades soluble and insoluble elastin. It may play a role in aneurysm formation and studies in mice suggest a role in the development of emphysema. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the center region of human MMP12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | HME antibody MGC138506 antibody MME antibody MMP12 antibody macrophage metalloelastase antibody matrix metalloproteinase 12 (macrophage elastase) antibody macrophage elastase antibody matrix metalloproteinase-12 antibody matrix metallopeptidase 12 |
|---|---|
| Molecular Weight(MW) | 54 kDa (note) |
| Cellular Localization | Secreted , extracellular space , extracellular matrix |
Application
MMP12 antibody [N3C1], Internal detects MMP12 protein at cytoplasm in human lung adenocarcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human lung adenocarcinoma. MMP12 antibody [N3C1], Internal diluted at 1:500.
Application
Immunofluorescence analysis of methanol-fixed H1299, using MMP12 antibody at 1:500 dilution.
Application
Immunohistochemical analysis of paraffin-embedded DLD1 xenograft, using MMP12 antibody at 1:500 dilution.
Application
Sample(30 µg whole cell lysate) A:293T 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.| Positive Control | 293T , H1299 , HelaS3 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
