Application
Western blot analysis of human recombinant MMP-2 with Protein band was detected at ~72 kDa (A); the band was blocked by the immunizing peptide (B).| Product Name | MMP2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP2Matrix metalloproteinases (MMPs), also called matrixins, belong to a family of proteases that are essential for the breakdown of extracellular matrix (ECM). They are thus important in apoptosis, tumor cell growth, invasion, and metastasis; as well as angiogenesis and wound healing. Most of the MMPs contain common domain structures that include a signal sequence, a propeptide, a catalytic domain, and a hemopexin-like (Hpx) domain. MMP2 and MMP9, the two gelatinases, contain three additional repeats of a fibronectin type II-like domain, enabling them to bind to and break down collagens. |
| Immunogen | Synthetic peptide corresponding to C-terminus of MMP2 (Human). |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIPWB |
| For ELISA: Use at a concentration of 0.1 - 1.0μg/ml. For IP: Use at a concentration of 3.0 - 5.0 μg/extract from 10^6 cells. For WB: Use at a concentration of 0.5 - 2μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|
| Species Reactivity | HumanMouseDogRatBovine |
| Concentration | 0.2mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | TBE-1 MMP2 CLG4A P08253 CLG4 120360 MMPII 4313 MMP-II MONA TBE1 TBE 1 MMP II |
|---|---|
| Target | MMP2 |
| Tissue Specificity | This antibody is specific to MMP2, and does not cross react with MMP1, 3, 7, 8, 9, 10 or 16. |
Application
Western blot analysis of human recombinant MMP-2 with Protein band was detected at ~72 kDa (A); the band was blocked by the immunizing peptide (B).| Application Notes | For ELISA: Use at a concentration of 0.1 - 1.0μg/ml. For IP: Use at a concentration of 3.0 - 5.0 μg/extract from 10^6 cells. For WB: Use at a concentration of 0.5 - 2μg/ml. Optimal dilutions/concentrations should be determined by the researcher.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4, containing 0.05% sodium azide |
