| Product Name | MMP20 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP20Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development,reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and so the protein is thought to play a role in tooth enamel formation. The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. |
| Immunogen | Synthetic peptide corresponding to the propeptide region of human MMP20. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHCIPWB |
| ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using a concentrated cell culture media from a stimulated human cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with a band at 58 kDa (proenzyme). Ab 11633 also recognizes non-reduced MMP20. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MMP20 MMP-20 9313 MMP25 MT6-MMP O60882 MT-MMP6 Q9NPA2 MT6MMP 204700 608482 64386 MTMMP6 MMP 20 MT6 MMP MT MMP6 |
|---|---|
| Target | MMP20 |
| Tissue Specificity | Rabbit Anti-MMP20, Propeptide Region specifically binds to MMP20 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc). The propeptide region of MMP20 is shed during enzymatic activation; thus the antibody does not recognize the activated forms of MMP20 and can be used to differentiate between the zymogen (proenzyme) and the activated enzyme. By immunoblotting against the reduced protein, the antibody reacts with a band at 58 kDa (proenzyme). Anti-MMP20, propeptide region also recognizes non-reduced MMP20. |
| Cellular Localization | Secreted |
| Positive Control | Osteosarcoma cells, HeLa cells, and gingival fibroblasts |
|---|---|
| Application Notes | ELISA: Use at an assay dependent dilution. IHC: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using a concentrated cell culture media from a stimulated human cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with a band at 58 kDa (proenzyme). Ab 11633 also recognizes non-reduced MMP20. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 15mM Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
