| Product Name | MMP23 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP23Matrix Metalloproteinase 23 (MMP21, MMP22, MIFR2, CAMMP) was first described in tumor cells. MMP23 shares much of the same domain structure as the classical MMPs, but has a unique cysteine array. The cysteine switch that is found in the classical MMPs is lacking or has a different sequence in MMP23. MMP23 has a furin cleavage site and it is thought to be localized within the cell. MMP23 has been reported as splice variants with different truncations at its C-terminus. MMP23 is predominantly expressed in ovary, testis, and prostate indicating that it plays a specialized role in reproductive processes. The human MMP23 gene has the chromosomal location of 1p36.3. The chromosome 1 location is a unique position among all the MMP genes currently mapped. |
| Immunogen | Synthetic peptide (Human) Hinge. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-FIPWB |
| ELISA: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using a concentrated cell culture media from a stimulated human cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with bands at 58 kDa (proenzyme), 45 kDa (active), and a series of further cleaved active forms. Since MMP23 levels in quiescent cells and tissues are minimal, mitogen stimulation or protein concentration is often needed to visualize the bands by immunoblotting. Treatment of the cells with the phorbal ester TPA or IL2 stimulates the production of MMP23 in some cell types. In addition, cell types differ greatly in the quantity of MMP23 produced. Even though MMP23 is localized within the cell, it may also be seen in conditioned media from cells in culture. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | 603320 O75900 MMP23B 8510 MIFR-1 Q9UBR9 MIFR MMP23A MIFR1 603321 MMP22 MMP23 8511 MMP21 MIFR 1 |
|---|---|
| Target | MMP23 |
| Tissue Specificity | specifically binds to MMP23 and does not cross-react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.). The antibody recognizes the proform and active forms of MMP23. |
| Cellular Localization | Secreted |
| Application Notes | ELISA: Use at an assay dependent dilution. IHC-Fr: Use at an assay dependent dilution. IP: Use at an assay dependent dilution. WB: Use at a dilution of 1/1000, this dilution is determined using a concentrated cell culture media from a stimulated human cell line, an alkaline phosphatase conjugated secondary antibody and BCIP/NBT as the substrate. Higher antibody concentrations may be necessary for non-human samples. By blotting against the reduced protein, the antibody reacts with bands at 58 kDa (proenzyme), 45 kDa (active), and a series of further cleaved active forms. Since MMP23 levels in quiescent cells and tissues are minimal, mitogen stimulation or protein concentration is often needed to visualize the bands by immunoblotting. Treatment of the cells with the phorbal ester TPA or IL2 stimulates the production of MMP23 in some cell types. In addition, cell types differ greatly in the quantity of MMP23 produced. Even though MMP23 is localized within the cell, it may also be seen in conditioned media from cells in culture. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Upon Receipt - Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.1% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
