| Product Name | MMP25 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MMP25Matrix Metalloproteinase 25, also known as membrane type matrix metalloproteinase 6 and leukolysin, was initially cloned from human leukocytes and found to be specifically expressed by peripheral blood leukocytes. The expression of MMP25 (leukolysin) at the mRNA level appears to be localized to neutrophils. MMP25 was also found in lung, spleen, brain tumors, and several tumor cell lines. Like the other membrane-type metalloproteinases, MMP25 is associated with the cell membrane, but is anchored to the cell surface via a GPI (glycosyl-phosphatidyl inositol) attachment, rather than through a transmembrane domain. MMP25 is proteolytically active and truncated forms digest gelatin in zymograms. Like the other MT MMPs, MMP25 has a furin cleavage site, where prohormone convertases can activate the zymogen. MMP25 can be shed from the from the cell surface, into the culture media under stimulatory conditions. MMP25 is expressed in brain tumors and may facilitate tumor progression through its ability to activate progelatinase A at the cell membrane in colon carcinomas or brain tumors. MMP25 may also play a role in the cellular migration and invasion of the extracellular matrix and basement membranes. The MMP25 gene maps to human chromosome 16p13.3. |
| Immunogen | Synthetic peptide (Human) propeptide. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. Treatment of cells with concanavolin A or the phorbol ester TPA stimulates production of MMP25 in some cell types, and the enzyme can be recovered in cell lysates. By immunoblotting against the reduced protein, the antibody identifies bands at 65 kDa, as well as breakdown products. The propeptide domain is removed upon activation thus the antibody recognizes the zymogen, and not the activated forms of the enzyme. Note: Higher antibody dilutions may be necessary for non-human samples. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | Human |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MMP20 MMP25 MT6-MMP MT-MMP6 Q9NPA2 MT6MMP 608482 64386 MTMMP6 MT6 MMP MT MMP6 |
|---|---|
| Target | MMP25 |
| Tissue Specificity | The antibody binds to the propeptide domain of MMP25 and does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.). |
| Cellular Localization | Secreted |
| Application Notes | WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. Treatment of cells with concanavolin A or the phorbol ester TPA stimulates production of MMP25 in some cell types, and the enzyme can be recovered in cell lysates. By immunoblotting against the reduced protein, the antibody identifies bands at 65 kDa, as well as breakdown products. The propeptide domain is removed upon activation thus the antibody recognizes the zymogen, and not the activated forms of the enzyme. Note: Higher antibody dilutions may be necessary for non-human samples. Optimal dilutions/concentrations should be determined by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Store at 4ºC short term. For extended storage aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: 50% Glycerol, 0.01M PBS. pH 7.4 |
