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WB: Use at a dilution of 1/1000. A starting dilution of 1/5000 is recommended for chemiluminescent substrates. Note: Higher antibody dilutions may be necessary for non-human samples. By immunoblotting against the reduced protein, the antibody identifies bands at 74 kDa and 58 kDa, as well as bands at 53 kDa and 50 kDa. The larger band may possibly represent an alternatively spliced form of MMP27. The antibody specifically binds to MMP27 and does not cross react with the other MMP family members (MMP1, MMP2, MMP3, MMP9, etc.). Optimal dilutions/concentrations should be determined by the end user. |