Application
Western blot analysis of MMP-9 expression in Jurkat cell lysate(Lane 1,2),Mouse 3T3 cell lysate(Lane3) and Rat kidney tissue lysate(Lane4).| Product Name | MMP9 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MMP-9The matrix metalloproteinases (MMP) are a family of peptidase enzymes responsible for the degradation of extracellular matrix components, including collagen, gelatin, fibronectin, laminin and proteoglycan. Transcription of MMP genes is differentially activated by phorbol ester, lipopolysaccharide (LPS) or staphylococcal enterotoxin B (SEB). MMP catalysis requires both calcium and zinc. MMP-9 (also designated 92-kDa type IV collagenase or gelatinase B) has been shown to degrade bone collagens in concert with MMP-1 (also designated interstitial collagenase, fibroblast collagenase or collagenase-1), and cysteine proteases and may play a role in bone osteoclastic resorption. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the full length (proenzyme, 92 kDa) and cleaved (active enzyme, 84 kDa) MMP-9 in samples from human, mouse, and rat origins.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | GELB EC3.4.24.35 EC 3.4.24.35 CLG4B Matrix metalloproteinase MMP9 |
|---|---|
| Target | MMP-9 |
| Tissue Specificity | This antibody is specific for MMP-9. |
Application
Western blot analysis of MMP-9 expression in Jurkat cell lysate(Lane 1,2),Mouse 3T3 cell lysate(Lane3) and Rat kidney tissue lysate(Lane4).| Application Notes | Western blotting (0.5-4 µg/ml). However, the optimal concentrations should be determined individually. The antibody recognizes the full length (proenzyme, 92 kDa) and cleaved (active enzyme, 84 kDa) MMP-9 in samples from human, mouse, and rat origins.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20ºC.The antibody solution should be gently mixed before use. |
| Storage Buffer | PBS (pH 7.2) containing 30% glycerol, 0.5% BSA, and 0.01% thimerosal |
