Application
WB analysis of U937 lysate (10 μg) using MMP9 antibody [EP1255Y] at a dilution of 1:1000.| Product Name | MMP9 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit monoclonal antibody [EP1255Y] to MMP-992-kDa Type IV collagenase (MMP-9), a member of matrix metalloproteinases, is believed to play a critical role in physiological tissue-remodeling processes and also in many pathological conditions such as tumor invasion. The induction of proteolytic enzymes is an important mechanism in the migration of monocytes into tissues and body fluids . MMP-9 is secreted from neutrophils, macrophages, and a number of transformed cells in zymogen form . MMP-9/gelatinase B is upregulated in angiogenic dysplasias and invasive cancers of the epidermis in a mouse model of multi-stage tumorigenesis elicited by HPV16 oncogenes. MMP-9 is predominantly expressed in neutrophils, macrophages, and mast cells, rather than in oncogene-positive neoplastic cells . |
| Immunogen | A synthetic peptide corresponding to residues near the C-terminus of human MMP-9 was used as an immunogen. |
| Clonality | Monoclonal |
|---|---|
| Isotype | IgG |
| Clone Number | EP1255Y |
| Host Species | Rabbit |
| Tested Applications | IHC-FIHC-PWB |
| WB: 1/1000. IHC-Fr: Use at an assay dependent concentration. IHC-P: 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Is unsuitable for Flow Cyt,ICC/IF or IP.: |
|
| Species Reactivity | HumanDog |
| Concentration | 1mg/ml |
| Purification | Protein A |
| Gene Synonyms | MMP-9 GELB CLG4B MANDP2 MMP9 MMP 9 |
|---|---|
| Target | MMP-9 |
| Molecular Weight(MW) | 78 kDa (note) |
Application
WB analysis of U937 lysate (10 μg) using MMP9 antibody [EP1255Y] at a dilution of 1:1000.| Application Notes | WB: 1/1000. IHC-Fr: Use at an assay dependent concentration. IHC-P: 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. Is unsuitable for Flow Cyt,ICC/IF or IP.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 °C. Stable for 12 months from date of receipt. |
| Storage Buffer | 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. |
