Application
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MNK1 (phospho Thr255) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MNK1 (phospho Thr255)This gene encodes a Ser/Thr protein kinase that interacts with, and is activated by ERK1 and p38 mitogen-activated protein kinases, and thus may play a role in the response to environmental stress and cytokines. This kinase may also regulate transcription by phosphorylating eIF4E via interaction with the C-terminal region of eIF4G. Alternatively spliced transcript variants have been noted for this gene. [provided by RefSeq, Jan 2012] |
| Immunogen | Peptide sequence around phosphorylation site of threonine 255 (L-T-T(p)-P-C) derived from Human MNK1. |
| Modification | p-Thr255 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: 1:500 - 1:3000: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MKNK1 MAP kinase interacting serine/threonine kinase 1 MNK1 |
|---|---|
| Target | MNK1 (phospho Thr255) |
| Tissue Specificity | The antibody detects endogenous levels of MNK1 only when phosphorylated at threonine 255. |
Application
Western blot analysis of extracts from HeLa cells, treated with Adriamycin (0.5ug/ml, 24hours), using MNK1 (Phospho-Thr255) antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | WB: 1:500 - 1:3000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20˚C |
| Storage Buffer | Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
