Application
MOBKL1B Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.Application
MOBKL1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOBKL1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.Application
Western blot analysis of MOBKL1B antibody (C-term) in A2058 cell line lysates (35ug/lane). MOBKL1B (arrow) was detected using the purified Pab.Application
Western blot analysis of MOBKL1B (arrow) using rabbit polyclonal MOBKL1B Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.| Product Name | MOBKL1B antibody, C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MOBKL1B (C-term)Activator of LATS1/2 in the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS1/2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. Stimulates the kinase activity of STK38 and STK38L. |
| Immunogen | KLH conjugated synthetic peptide between 187~216 amino acids from the C-terminal region of human MOBKL1B. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | FACSIHC-PWB |
| ELISA: Use at 1:1,000: |
|
| Species Reactivity | Human |
| Concentration | 0.2mg/ml |
| Gene Synonyms | Mob1 alpha Mps one binder kinase activator-like 1B Mps one binder kinase activatorlike 1B MOBKL1B Mob1 homolog1B MOBK1B Mob1 homolog 1B Mob1A Mps one binder kinase activatorlike1B MOB4B C2orf6 Protein Mob4B |
|---|---|
| Target | MOBKL1B |
| Molecular Weight(MW) | 25.08 kDa (note) |
Application
MOBKL1B Antibody (C-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Application
MOBKL1B Antibody (C-term) IHC analysis in formalin fixed and paraffin embedded human Prostate carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the MOBKL1B Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.
Application
Western blot analysis of MOBKL1B antibody (C-term) in A2058 cell line lysates (35ug/lane). MOBKL1B (arrow) was detected using the purified Pab.
Application
Western blot analysis of MOBKL1B (arrow) using rabbit polyclonal MOBKL1B Antibody (C-term) . 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MOBKL1B gene.| Application Notes | ELISA: Use at 1:1,000: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at at 2-8°C for up to 6 months. For long term storage, aliquot and store at -20 °C. Avoid repeated freeze/thaw cycles. |
| Storage Buffer | PBS with 0.09% (W/V) sodium azide |
