Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N1N3] diluted at 1:3000.Application
Immunofluorescence analysis of methanol-fixed HepG2, using Monoamine Oxidase B antibody at 1:200 dilution.Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Monoamine Oxidase B antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:3000| Product Name | Monoamine Oxidase B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Monoamine Oxidase B (monoamine oxidase B)The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanRat |
| Predicted Reactivity | Mouse, Dog, Pig, Bovine, Guinea pigMouse (88%), Dog (93%), Pig (90%), Bovine (90%), Guinea pig (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC26382 antibody, MAOB antibody, "MAO, platelet antibody", monoamine oxidase type B antibody, adrenalin oxidase antibody, tyramine oxidase antibody, "MAO, brain antibody", MAO-B antibody, amine oxidase [flavin-containing] B antibody, monoamine oxidase B a |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) (2) |
| Cellular Localization | Mitochondrion outer membrane |
Application
Rat tissue extract (50 μg) was separated by 10% SDS-PAGE, and the membrane was blotted with Monoamine Oxidase B antibody [N1N3] diluted at 1:3000.
Application
Immunofluorescence analysis of methanol-fixed HepG2, using Monoamine Oxidase B antibody at 1:200 dilution.
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using Monoamine Oxidase B antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: Hep G2 7.5% SDS PAGE diluted at 1:3000| Positive Control | HepG2 , Rat liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
