Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Monoamine Oxidase B antibody at 1:100 dilution.Application
Sample (50 µg of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Sample(30 µg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
ELISA detection of MAOB using for capture at a concentration of 2.5 µg/mL and for detection at a concentration of 0.5 µg/mL.Application
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500.Application
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B protein stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | Monoamine Oxidase B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Monoamine Oxidase B (monoamine oxidase B)The protein encoded by this gene belongs to the flavin monoamine oxidase family. It is a enzyme located in the mitochondrial outer membrane. It catalyzes the oxidative deamination of biogenic and xenobiotic amines and plays an important role in the metabolism of neuroactive and vasoactive amines in the central nervous sysytem and peripheral tissues. This protein preferentially degrades benzylamine and phenylethylamine. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Monoamine Oxidase B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ELISAIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Dog, Pig, Bovine, Guinea pigRat (86%), Dog (94%), Pig (90%), Bovine (90%), Guinea pig (86%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MGC26382 antibody, MAOB antibody, "MAO, platelet antibody", monoamine oxidase type B antibody, adrenalin oxidase antibody, tyramine oxidase antibody, "MAO, brain antibody", MAO-B antibody, amine oxidase [flavin-containing] B antibody, monoamine oxidase B a |
|---|---|
| Molecular Weight(MW) | 59 kDa (note) |
| Cellular Localization | Mitochondrion outer membrane |
Application
Immunohistochemical analysis of paraffin-embedded OVCA xenograft, using Monoamine Oxidase B antibody at 1:100 dilution.
Application
Sample (50 µg of whole cell lysate) A: Mouse liver 7.5% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Sample(30 µg of whole cell lysate) A:Hep G2 7.5% SDS PAGE diluted at 1:1500 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
ELISA detection of MAOB using for capture at a concentration of 2.5 µg/mL and for detection at a concentration of 0.5 µg/mL.
Application
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at cytoplasm on mouse lung by immunohistochemical analysis. Sample: Paraffin-embedded mouse lung. Monoamine Oxidase B antibody [N2C3] diluted at 1:500.
Application
Monoamine Oxidase B antibody [N2C3] detects Monoamine Oxidase B protein at mitochondria by immunofluorescent analysis. Sample: HepG2 cells were fixed in ice-cold MeOH for 5 min. Green: Monoamine Oxidase B protein stained by Monoamine Oxidase B antibody [N2C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | HepG2 , mouse liver |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
