Application
Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 7.5% SDS PAGE diluted at 1:500Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MPP5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Product Name | MPP5 antibody , N-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MPP5 (membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5))Members of the peripheral membrane-associated guanylate kinase (MAGUK) family function in tumor suppression and receptor clustering by forming multiprotein complexes containing distinct sets of transmembrane, cytoskeletal, and cytoplasmic signaling proteins. All MAGUKs contain a PDZ-SH3-GUK core and are divided into 4 subfamilies, DLG-like (see DLG1; MIM 601014), ZO1-like (see TJP1; MIM 601009), p55-like (see MPP1; MIM 305360), and LIN2-like (see CASK; MIM 300172), based on their size and the presence of additional domains (Tseng et al., 2001 [PubMed 11311936]). MPP5 is a member of the p55-like MAGUK subfamily.[supplied by OMIM] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human MPP5. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | BovineBovine (100%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ12615 antibody, PALS1 antibody, MPP5 antibody, stardust antibody, MAGUK p55 subfamily member 5 antibody, "membrane protein, palmitoylated 5 (MAGUK p55 subfamily member 5) antibody" |
|---|---|
| Molecular Weight(MW) | 77 kDa (note) (2) |
| Cellular Localization | Cell membrane; Peripheral membrane protein , Endomembrane system; Peripheral membrane protein , Cell junction , tight junction |
Application
Sample (30 ug of whole cell lysate) A: U87-MG B: SK-N-SH 7.5% SDS PAGE diluted at 1:500
Application
Confocal immunofluorescence analysis (Olympus FV10i) of methanol-fixed HeLa, using MPP5 antibody (Green) at 1:500 dilution. Alpha-tubulin filaments were labeled with (Red) at 1:2000.| Positive Control | U87-MG , SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 40% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
