Application
Immunocytochemistry/Immunofluorescence: Mre11 Antibody [ - Detection of MRE11 (Green) in Hela cells using at a 1:200 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.Application
Immunohistochemistry: Mre11 Antibody [ - MRE11 staining in the human epidermis detected withApplication
Western Blot: Mre11 Antibody [ - Analysis of Mre11 on 50 ug of HeLa and MEF lysates, usingApplication
Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells. (Panel D - negative control). Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.| Product Name | Mre11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Mre11The hMre11-hRad50-NBS1 protein plays a central role in the human cellular DNA-Damage response, with recent studies indicating that these proteins help link DNA-damage detection to DNA-repair and cell cycle-checkpoint functions. This protein complex has been implicated in the activation of cell cycle-regulatory pathways, due to the fact that the NBS1 gene mutates in the chromosomal-instability syndrome, Nijmegen breakdown syndrome (NBS), which is characterized by increased cancer incidence, cell cycle checkpoint defects, and ionizing radiation sensitivity. hMre11-hRad50 foci form in response to DNA double-strand breaks and rely upon a DNA damage-induced signaling pathway. However, hMre11 migrates to sites of damage while hRad51 does not localize at these sites. These findings are consistent with the distinct role of these proteins in DNA repair. |
| Immunogen | Human Mre11 full length. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIPWB |
| By Western blot, this antibody recognizes a band at 81 kDa representing hMre11 in TK6 or other human cells. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 10^6 cells. Suggested working dilutions: Western Blot: 1:10,000 in human and mouse Immunofluorescence: 1:200 in human (has not been tested in mouse) Immunoprecipitation: 3 ul for 3 x 10-6 cells (in human and mouse): |
|
| Species Reactivity | HumanMouse |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRE11 MRE11B P49959 600814 ATLD 4361 MRE11A HNGS1 |
|---|---|
| Target | Mre11 |
| Tissue Specificity | This antibody is specific for human Mre11 (full length). |
Application
Immunocytochemistry/Immunofluorescence: Mre11 Antibody [ - Detection of MRE11 (Green) in Hela cells using at a 1:200 dilution. Nuclei (Blue) are counterstained using Hoechst 33258.
Application
Immunohistochemistry: Mre11 Antibody [ - MRE11 staining in the human epidermis detected with
Application
Western Blot: Mre11 Antibody [ - Analysis of Mre11 on 50 ug of HeLa and MEF lysates, using
Application
Indirect immunofluorescence to detect localisation of Mre11 in normal lymphoblastoid cells. (Panel D - negative control). Image supplied by Dr Domenico Delia, Istituto Nazionale Tumori, Italy.| Positive Control | Hela whole cell lysate |
|---|---|
| Application Notes | By Western blot, this antibody recognizes a band at 81 kDa representing hMre11 in TK6 or other human cells. By immunofluorescence, this antibody has been used with methanol-fixed IMR90 primary human fibroblasts. Immunoprecipitation tests have been done with 3-4 X 10^6 cells. Suggested working dilutions: Western Blot: 1:10,000 in human and mouse Immunofluorescence: 1:200 in human (has not been tested in mouse) Immunoprecipitation: 3 ul for 3 x 10-6 cells (in human and mouse): |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. |
| Storage Buffer | 0.02% sodium azide. |
