Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using MRPL10 antibody at 1:500 dilution.| Product Name | MRPL10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL10 (mitochondrial ribosomal protein L10)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found on chromosome 5q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (83%), Rat (86%), Rhesus Monkey (97%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | L10MT antibody, MGC17973 antibody, MRP-L10 antibody, MRP-L8 antibody, MRPL8 antibody, RPML8 antibody, MRPL10 antibody, "39S ribosomal protein L8, mitochondrial antibody", L8mt antibody, "39S ribosomal protein L10, mitochondrial antibody", mitochondrial rib |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (3) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: 293T 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded Mahlarvu xenograft, using MRPL10 antibody at 1:500 dilution.| Positive Control | 293T |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
