Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
MRPL11 antibody [N1C3] detects MRPL11 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. MRPL11 antibody [N1C3] dilution: 1:250.| Product Name | MRPL11 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL11 (mitochondrial ribosomal protein L11)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. Sequence analysis identified three transcript variants that encode different isoforms. Pseudogenes corresponding to this gene are found on chromosomes 5q and 12q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL11. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (89%), Rat (81%), Rhesus Monkey (98%), Bovine (87%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | L11MT antibody, MGC111024 antibody, MRP-L11 antibody, MRPL11 antibody, "39S ribosomal protein L11, mitochondrial antibody", mitochondrial ribosomal protein L11 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (3) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
MRPL11 antibody [N1C3] detects MRPL11 protein at cytoplasm on human lung carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded lung carcinoma. MRPL11 antibody [N1C3] dilution: 1:250.| Positive Control | 293T , A431 , Jurakt , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 1XPBS, 1% BSA, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
