Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12 antibody at 1:250 dilution.Application
MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: MRPL12 protein stained by MRPL12 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Product Name | MRPL12 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL12 (mitochondrial ribosomal protein L12)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein which forms homodimers. In prokaryotic ribosomes, two L7/L12 dimers and one L10 protein form the L8 protein complex. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL12. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | HumanMouse |
| Predicted Reactivity | Rat, Rhesus Monkey, BovineRat (83%), Rhesus Monkey (94%), Bovine (86%) |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 5c5-2 antibody, FLJ60124 antibody, L12mt antibody, MGC8610 antibody, MRP-L31/34 antibody, MRPL7 antibody, MRPL7/L12 antibody, RPML12 antibody, MRPL12 antibody, MRP-L12 antibody, "39S ribosomal protein L12, mitochondrial antibody", mitochondrial ribosomal p |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (50 ug of whole cell lysate) A: mouse brain 12% SDS PAGE diluted at 1:1000
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000
Application
Immunohistochemical analysis of paraffin-embedded human breast cancer, using MRPL12 antibody at 1:250 dilution.
Application
MRPL12 antibody [N1C3] detects MRPL12 protein at mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in 2% paraformaldehyde/culture medium at 37℃ for 30 min. Green: MRPL12 protein stained by MRPL12 antibody [N1C3] diluted at 1:500. Blue: Hoechst 33342 staining.| Positive Control | A431 , H1299 , HeLa , HepG2 , mouse brain |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
