Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL18 antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG 12% SDS PAGE diluted at 1:1000| Product Name | MRPL18 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL18 (39S ribosomal protein L18, mitochondrial)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L18P ribosomal protein family. Three polymorphic sites exist in this gene, one of which is three nt in length which causes an extra aa near the N-terminus. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL18. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Rhesus Monkey, BovineMouse (86%), Rat (84%), Rhesus Monkey (97%), Bovine (88%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | L18mt antibody, MRP-L18 antibody, MRPL18 antibody, "39S ribosomal protein L18, mitochondrial antibody", mitochondrial ribosomal protein L18 antibody |
|---|---|
| Molecular Weight(MW) | 21 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL18 antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: NT2D1 B: PC-3 C: U87-MG 12% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , PC-3 , U87-MG |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
