Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL28 antibody at 1:250 dilution.Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000| Product Name | MRPL28 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL28 (mitochondrial ribosomal protein L28)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein, a part of which was originally isolated by its ability to recognize tyrosinase in an HLA-A24-restricted fashion. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL28. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (89%), Bovine (89%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MAAT1 antibody, MGC8499 antibody, p15 antibody, MRPL28 antibody, L28mt antibody, melanoma antigen p15 antibody, melanoma-associated antigen recognised by cytotoxic T lymphocytes antibody, melanoma-associated antigen recognized by T lymphocytes antibody, "3 |
|---|---|
| Molecular Weight(MW) | 30 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPL28 antibody at 1:250 dilution.
Application
Sample (30 ug of whole cell lysate) A: HepG2 12% SDS PAGE diluted at 1:3000| Positive Control | A549 , HeLa , HepG2 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
