Application
Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE diluted at 1:1000| Product Name | MRPL3 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPL3 (mitochondrial ribosomal protein L3)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein that belongs to the L3P ribosomal protein family. A pseudogene corresponding to this gene is found on chromosome 13q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPL3. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Xenopus laevis, Chicken, Chimpanzee, BovineMouse (90%), Rat (89%), Xenopus laevis (80%), Chicken (80%), Chimpanzee (96%), Bovine (87%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MRL3 antibody, RPML3 antibody, MRPL3 antibody, "39S ribosomal protein L3, mitochondrial antibody", L3mt antibody, mitochondrial 60S ribosomal protein L3 antibody, MRP-L3 antibody, mitochondrial ribosomal protein L3 antibody |
|---|---|
| Molecular Weight(MW) | 39 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: A549 B: HeLa 10% SDS PAGE diluted at 1:1000| Positive Control | A549 , HeLa , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
