Rabbit Polyclonal antibody to MRPL9 (mitochondrial ribosomal protein L9)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 39S subunit protein. A pseudogene corresponding to this gene is found at 8q21.11. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the center region of human MRPL9. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Rhesus MonkeyMouse (90%), Rat (92%), Rhesus Monkey (96%)
Concentration
1mg/ml (Please refer to the vial label for the specific concentration)
Purification
Affinity purified
Target Information
Gene Synonyms
L9mt antibody MRPL9 antibody MRP-L9 antibody "39S ribosomal protein L9 mitochondrial antibody" mitochondrial ribosomal protein L9 antibody
Molecular Weight(MW)
30 kDa (note) (2)
Application
Application
Sample (30 ug of whole cell lysate) A: Hep G2 12% SDS PAGE diluted at 1:1000
Positive Control
A549 , HeLa , HepG2 , HCT116
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.