Application
Immunofluorescence analysis of methanol-fixed MCF-7, using MRPS10 antibody at 1:200 dilution.Application
MRPS10 antibody [N1C3] detects MRPS10 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: MRPS10 antibody [N1C3] dilution: 1:250.Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000| Product Name | MRPS10 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPS10 (mitochondrial ribosomal protein S10)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S10P family. Pseudogenes corresponding to this gene are found on chromosomes 1q, 3p, and 9p. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPS10. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, BovineMouse (88%), Bovine (93%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10567 antibody, MRP-S10 antibody, PNAS-122 antibody, MRPS10 antibody, mitochondrial 28S ribosomal protein S10 antibody, "28S ribosomal protein S10, mitochondrial antibody", S10mt antibody, mitochondrial ribosomal protein S10 antibody |
|---|---|
| Molecular Weight(MW) | 23 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunofluorescence analysis of methanol-fixed MCF-7, using MRPS10 antibody at 1:200 dilution.
Application
MRPS10 antibody [N1C3] detects MRPS10 protein at cytosol on human breast carcinoma by immunohistochemical analysis. Sample: MRPS10 antibody [N1C3] dilution: 1:250.
Application
Sample (30 ug of whole cell lysate) A: IMR32 12% SDS PAGE diluted at 1:1000| Positive Control | NT2D1 , IMR32 , U87-MG , MCF-7 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
