Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPS18B antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Product Name | MRPS18B antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPS18B (mitochondrial ribosomal protein S18B)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that belongs to the ribosomal protein S18P family. The encoded protein is one of three that has significant sequence similarity to bacterial S18 proteins. The primary sequences of the three human mitochondrial S18 proteins are no more closely related to each other than they are to the prokaryotic S18 proteins. Pseudogenes corresponding to this gene are found on chromosomes 1q and 2q. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPS18B. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher. | |
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Pig, Rhesus Monkey, Chimpanzee, BovineMouse (85%), Rat (85%), Pig (90%), Rhesus Monkey (94%), Chimpanzee (98%), Bovine (90%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | C6orf14 antibody, DKFZp564H0223 antibody, HSPC183 antibody, HumanS18a antibody, MRP-S18-2 antibody, MRPS18-2 antibody, PTD017 antibody, S18amt antibody, MRPS18B antibody, S18mt-b antibody, "28S ribosomal protein S18-2, mitochondrial antibody", "28S ribosom |
|---|---|
| Molecular Weight(MW) | 29 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using MRPS18B antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: A431 12% SDS PAGE diluted at 1:1000| Positive Control | A431 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher. |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
