Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using MRPS22 antibody at 1:300 dilution.Application
Sample(30 ug whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000| Product Name | MRPS22 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MRPS22 (mitochondrial ribosomal protein S22)Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that does not seem to have a counterpart in prokaryotic and fungal-mitochondrial ribosomes. This gene lies telomeric of and is transcribed in the opposite direction from the forkhead box L2 gene. A pseudogene corresponding to this gene is found on chromosome Xq. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MRPS22. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (85%), Rat (85%), Bovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | C3orf5 antibody COXPD5 antibody GIBT antibody MRP-S22 antibody RPMS22 antibody MRPS22 antibody S22mt antibody "28S ribosomal protein S22 mitochondrial antibody" mitochondrial ribosomal protein S22 antibody |
|---|---|
| Molecular Weight(MW) | 41 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunohistochemical analysis of paraffin-embedded human lung SCC, using MRPS22 antibody at 1:300 dilution.
Application
Sample(30 ug whole cell lysate) A:HeLa S3 10% SDS PAGE diluted at 1:1000| Positive Control | 293T , A431 , H1299 , HeLaS3 , HepG2 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
