Rabbit polyclonal antibody to MRPS30Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that is similar to the chicken pro-apoptotic protein p52. Transcript variants using alternative promoters or polyA sites have been mentioned in the literature but the complete description of these sequences is not available. [provided by RefSeq]
Immunogen
The antiserum was produced against synthesized peptide derived from human MRPS30.
Key Feature
Clonality
Polyclonal
Host Species
Rabbit
Tested Applications
ELISAWB
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Species Reactivity
Human
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
Mitochondrial28S ribosomal protein S30 MRPS30 Programmed cell death protein9 S30mt MRP-S30 Mitochondrial 28S ribosomal protein S30 Programmed cell death protein 9 MRP S30
Target
MRPS30
Molecular Weight(MW)
50 kDa (note)
Tissue Specificity
MRPS30 Antibody detects endogenous levels of total MRPS30 protein.
Application
Application
Western blot analysis of extracts from LOVO cells, using MRPS30 Antibody. The lane on the right is treated with the synthesized peptide.
Application Notes
ELISA: 1:10000Optimal dilutions should be determined experimentally by the end user.:
Additional Information
Form
Liquid
Storage Instructions
Store at -20 degrees Celcius. Product is stable for at least 1 year.
Storage Buffer
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.