Application
Western blot analysis of extracts from Jurkat/COLO/A549 cells, using MRPS35 Antibody. The lane on the right is treated with the synthesized peptide.| Product Name | MRPS35 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MRPS35Mammalian mitochondrial ribosomal proteins are encoded by nuclear genes and help in protein synthesis within the mitochondrion. Mitochondrial ribosomes (mitoribosomes) consist of a small 28S subunit and a large 39S subunit. They have an estimated 75% protein to rRNA composition compared to prokaryotic ribosomes, where this ratio is reversed. Another difference between mammalian mitoribosomes and prokaryotic ribosomes is that the latter contain a 5S rRNA. Among different species, the proteins comprising the mitoribosome differ greatly in sequence, and sometimes in biochemical properties, which prevents easy recognition by sequence homology. This gene encodes a 28S subunit protein that has had confusing nomenclature in the literature. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. Pseudogenes corresponding to this gene are found on chromosomes 3p, 5q, and 10q. [provided by RefSeq] |
| Immunogen | The antiserum was produced against synthesized peptide derived from human MRPS35. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ELISAWB |
| ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
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| Species Reactivity | Human |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | Mitochondrial ribosomal protein S28 S35mt mitochondrial MRP-S35 MRPS28 MRP-S28 28S ribosomal protein S35 MRPS35 MRP S35 MRP S28 |
|---|---|
| Target | MRPS35 |
| Molecular Weight(MW) | 36 kDa (note) |
| Tissue Specificity | MRPS35 Antibody detects endogenous levels of total MRPS35 protein. |
Application
Western blot analysis of extracts from Jurkat/COLO/A549 cells, using MRPS35 Antibody. The lane on the right is treated with the synthesized peptide.| Application Notes | ELISA: 1:40000Optimal dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20 degrees Celcius. Product is stable for at least 1 year. |
| Storage Buffer | Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
