Rabbit Polyclonal antibody to MSH2 (mutS homolog 2, colon cancer, nonpolyposis type 1 (E. coli))MSH2 was identified as a locus frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. [provided by RefSeq]
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human MSH2. The exact sequence is proprietary.
Key Feature
Clonality
Polyclonal
Isotype
IgG
Host Species
Rabbit
Tested Applications
WB
*Optimal dilutions/concentrations should be determined by the researcher.
:
Species Reactivity
Human
Predicted Reactivity
Mouse, Rat, Pig, BovineMouse (93%), Rat (92%), Pig (93%), Bovine (95%)
Concentration
1mg/ml
Purification
Affinity purified
Target Information
Gene Synonyms
COCA1 antibody FCC1 antibody HNPCC antibody HNPCC1 antibody LCFS2 antibody MSH2 antibody DNA mismatch repair protein Msh2 antibody mutS protein homolog 2 antibody hMSH2 antibody "mutS homolog 2 colon cancer nonpolyposis type 1 (E. coli) antibody
Molecular Weight(MW)
105 kDa (note) (2)
Cellular Localization
Nucleus
Application
Application
Sample (30 ug of whole cell lysate) A: 293T B: A431 7.5% SDS PAGE diluted at 1:1000
Positive Control
293T , A431 , Raji
Application Notes
*Optimal dilutions/concentrations should be determined by the researcher.
:
Additional Information
Form
Liquid
Storage Instructions
Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles.
Storage Buffer
0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative.