| Product Name | MSK1 (phospho Thr581) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MSK1 (phospho Thr581)MSK1, a Mitogen and Stress activated protein Kinase, is activated by ERK as well as p38 MAPK in response to growth factors and various cellular stress stimuli. MSK1 resembles RSK by having two kinase domains connected by a regulator linker region. Phosphorylation of RSK1 at Ser 364, Ser381 and Thr574 is critical for RSK1 activity. These sites are analogous to Ser360, Ser376 and Thr581 of MSK1 and maybe important for MSK1 activity as well. |
| Modification | p-Thr581 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| Western blot (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Phospho-MSK1(Thr581) detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1, RSK2 or RSK3.: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | RLPK phospho-MSK1 (pThr581) phospho-MSK1 (pT581) phospho MSK1 (pT581) MSPK1 phosphoMSK1 (pThr581) phospho MSK1 (pThr581) RSKL MGC1911 MSK1 RPS6KA5 phosphoMSK1 (pT581) MSK1 (phospho T581) MSK1 (phospho Thr581) MSK1 (pT581) MSK1 (pThr581) MSK |
|---|---|
| Target | MSK1 (phospho Thr581) |
| Tissue Specificity | This antibody is specific for MSK1 (phospho Thr581). |
| Cellular Localization | Predominantly nuclear. Partially cytoplasmic. |
| Application Notes | Western blot (0.5-4 µg/ml). However, the optimal conditions should be determined individually. Phospho-MSK1(Thr581) detects 90 kDa MSK1 only when phosphorylated at Thr581. The antibody does not cross-react with nonphosphorylated MSK1 or MSK1 phosphorylated at other sites. It does not cross-react with RSK1, RSK2 or RSK3.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | The antibody solution should be gently mixed before use. Store at +4C. Product should remain stable for at least 12 months. |
| Storage Buffer | PBS (pH 7.2) containing 50% glycerol, 1% BSA, 0.02% thimerosal |
