Application
Western blot analysis of extracts from HUVEC cells using MSK1 Antibody and the same antibody preincubated with blocking peptide.Application
Western blot analysis of extracts from HUVEC cells using MSK1(Ab-376) Antibody and the same antibody preincubated with blocking peptide.| Product Name | MSK1 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MSK1Serine/threonine kinase required for the mitogen or stress-induced phosphorylation of the transcription factors CREB (cAMP response element-binding protein) and ATF1 (activating transcription factor-1). Essential role in the control of RELA transcriptional activity in response to TNF. Directly represses transcription via phosphorylation of 'Ser-1' of histone H2A. Phosphorylates 'Ser-10' of histone H3 in response to mitogenics, stress stimuli and epidemal growth-factor (EGF), which results in the transcriptional activation of several immediate early genes, including proto-oncogenes c-fos/FOS and c-jun/JUN. May also phosphorylate 'Ser-28' of histone H3. Mediates the mitogen- and stress-induced phosphorylation of high mobility group protein 14 (HMG-14). |
| Immunogen | Peptide sequence around aa.374~378 (G-Y-S-F-V) derived from Human MSK1. |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MGC1911 MSK1 MSPK1 RLPK RPS6KA5 RSK-like protein kinase ribosomal protein S6 kinase alpha-5 S6K-alpha-5 90 kDa ribosomal protein S6 kinase 5 nuclear mitogen- and stress-activated protein kinase 1 RSKL ribosomal protein S6 kinase 90kDa polypept |
|---|---|
| Target | MSK1 |
| Molecular Weight(MW) | 90 (note) |
Application
Western blot analysis of extracts from HUVEC cells using MSK1 Antibody and the same antibody preincubated with blocking peptide.
Application
Western blot analysis of extracts from HUVEC cells using MSK1(Ab-376) Antibody and the same antibody preincubated with blocking peptide.| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
