Application
Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] . Western blot analysis was performed using MTHFD2 antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.Application
MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] diluted at 1:500.Application
MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MTHFD2 antibody [N1C3] diluted at 1:500.Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2 antibody at 1:200 dilution.Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa E: HepG2 F: Molt-4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2 antibody at 1:500 dilution.| Product Name | MTHFD2 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MTHFD2 (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase)This gene encodes a nuclear-encoded mitochondrial bifunctional enzyme with methylenetetrahydrofolate dehydrogenase and methenyltetrahydrofolate cyclohydrolase activities. The enzyme functions as a homodimer and is unique in its absolute requirement for magnesium and inorganic phosphate. Formation of the enzyme-magnesium complex allows binding of NAD. Alternative splicing results in two different transcripts, one protein-coding and the other not protein-coding. This gene has a pseudogene on chromosome 7. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human MTHFD2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-PIPWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, Zebrafish, Xenopus laevis, Chicken, BovineMouse (95%), Rat (93%), Zebrafish (86%), Xenopus laevis (90%), Chicken (92%), Bovine (95%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | NMDMC antibody MTHFD2 antibody NAD-dependent methylene tetrahydrofolate dehydrogenase cyclohydrolase antibody "bifunctional methylenetetrahydrofolate dehydrogenase/cyclohydrolase mitochondrial antibody" "methylenetetrahydrofolate dehydrogenase (NADP+ |
|---|---|
| Molecular Weight(MW) | 38 kDa (note) |
| Cellular Localization | Mitochondrion |
Application
Immunoprecipitation of MTHFD2 protein from 293T whole cell extracts using 5 μg of MTHFD2 antibody [N1C3] . Western blot analysis was performed using MTHFD2 antibody [N1C3] . EasyBlot anti-Rabbit IgG was used as a secondary reagent.
Application
MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria by immunohistochemical analysis. Sample: Paraffin-embedded human MCF7 xenograft. MTHFD2 antibody [N1C3] diluted at 1:500.
Application
MTHFD2 antibody [N1C3] detects MTHFD2 protein at mitochondria in human breast carcinoma by immunohistochemical analysis. Sample: Paraffin-embedded human breast carcinoma. MTHFD2 antibody [N1C3] diluted at 1:500.
Application
Immunofluorescence analysis of paraformaldehyde-fixed HeLa, using MTHFD2 antibody at 1:200 dilution.
Application
Sample (30 µg of whole cell lysate) A: 293T B: A431 C: H1299 D: HeLa E: HepG2 F: Molt-4 G: Raji 10% SDS PAGE diluted at 1:1000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MTHFD2 antibody at 1:500 dilution.| Positive Control | 293T , A431 , H1299 , HeLa , HepG2 , Molt-4 , Raji , *SK-N-DZ , *SK-N-SH |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
