Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:3000Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mt PAP antibody [N1C1] diluted at 1:3000.| Product Name | mtPAP antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to mtPAP (mitochondrial poly(A) polymerase)The protein encoded by this gene is a member of the DNA polymerase type-B-like family. This enzyme synthesizes the 3' poly(A) tail of mitochondrial transcripts and plays a role in replication-dependent histone mRNA degradation. |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Mt PAP. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| *Optimal dilutions/concentrations should be determined by the researcher.
: |
|
| Species Reactivity | Human |
| Predicted Reactivity | PigPig (80%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | FLJ10486 antibody PAPD1 antibody SPAX4 antibody MTPAP antibody terminal uridylyltransferase 1 antibody TUTase 1 antibody PAP-associated domain-containing protein 1 antibody polynucleotide adenylyltransferase antibody "poly(A) RNA polymerase mitoch |
|---|---|
| Target | Mt PAP |
| Molecular Weight(MW) | 66 kDa (note) (2) |
| Cellular Localization | Mitochondrion |
Application
Sample (30 ug of whole cell lysate) A: HCT116 7.5% SDS PAGE diluted at 1:3000
Application
Various whole cell extracts (30 µg) were separated by 7.5% SDS-PAGE, and the membrane was blotted with Mt PAP antibody [N1C1] diluted at 1:3000.| Positive Control | A549 , HeLa , HepG2 , HCT116 , A549 , H1299 , HCT116 |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
Data sheet for OM264116