Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MUC2 antibody at 1:500 dilution.Application
Sample (30 µg whole cell lysate) A:Raji 5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.Application
MUC2 antibody [C3], C-term detects MUC2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MUC2 protein stained by MUC2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.Application
MUC2 antibody [C3], C-term detects MUC2 protein at secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MUC2 antibody [C3], C-term dilution: 1:500.| Product Name | MUC2 antibody , C-term |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MUC2 (mucin 2, oligomeric mucus/gel-forming)This gene encodes a member of the mucin protein family. Mucins are high molecular weight glycoproteins produced by many epithelial tissues. The protein encoded by this gene is secreted and forms an insoluble mucous barrier that protects the gut lumen. The protein polymerizes into a gel of which 80% is composed of oligosaccharide side chains by weight. The protein features a central domain containing tandem repeats rich in threonine and proline that varies between 50 and 115 copies in different individuals. Alternatively spliced transcript variants of this gene have been described, but their full-length nature is not known. [provided by RefSeq] |
| Immunogen | Carrier-protein conjugated synthetic peptide encompassing a sequence within the C-terminus region of human MUC2. The exact sequence is proprietary. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | HumanMouse |
| Predicted Reactivity | BovineBovine (86%) |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MLP antibody MUC-2 antibody SMUC antibody MUC2 antibody "mucin 2 intestinal/tracheal antibody" mucin-like protein antibody intestinal mucin-2 antibody mucin-2 antibody "mucin 2 oligomeric mucus/gel-forming antibody" |
|---|---|
| Molecular Weight(MW) | 540 kDa (note) |
| Cellular Localization | Secreted |
Application
Immunohistochemical analysis of paraffin-embedded SAS xenograft, using MUC2 antibody at 1:500 dilution.
Application
Sample (30 µg whole cell lysate) A:Raji 5% SDS PAGE diluted at 1:3000 The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
Application
MUC2 antibody [C3], C-term detects MUC2 protein at cytoplasm by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: MUC2 protein stained by MUC2 antibody [C3], C-term diluted at 1:500. Blue: Hoechst 33342 staining.
Application
MUC2 antibody [C3], C-term detects MUC2 protein at secreted on mouse intestine by immunohistochemical analysis. Sample: Paraffin-embedded mouse intestine. MUC2 antibody [C3], C-term dilution: 1:500.| Positive Control | H1299 , Molt-4 , Raji |
|---|---|
| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 10% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
