Application
Western blot analysis of MyD88 in Jurkat whole cell lysate with MyD88 antibody at 1:500 dilution.| Product Name | MyD88 antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MyD88The pro-inflammatory cytokine IL-1 induced cellular response requires IL-1 receptor complex including IL-1RI and IL-1RAcP. MyD88 is an adapter molecule in the IL-1 signaling pathway (1). MyD88 associates with and recruits IRAK to the IL-1 receptor complex in response to IL-1 treatment and dominant negative form of MyD88 attenuates IL-1R-mediated NF-kB activation. MyD88 is also a regulator molecule of IL-18 receptor and human Toll receptor (2,3). Targeted disruption of the MyD88 gene results in lose of cellular responses to IL-1 and IL-18, and MyD88-deficient mice lack responses to bacterial product LPS that employs Toll-like receptors 2 and 4 (TLR2 and TLR4) as the signaling receptors. |
| Immunogen | Synthetic peptide - amino acids 279-296 of MyD88 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher: |
|
| Species Reactivity | HumanMouse |
| Concentration | 0.5mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MYD88D |
|---|---|
| Target | MyD88 |
Application
Western blot analysis of MyD88 in Jurkat whole cell lysate with MyD88 antibody at 1:500 dilution.| Positive Control | Jurkat whole cell lysate |
|---|---|
| Application Notes | For WB: Use at a dilution of 1:500-1:1,000. Optimal dilutions/concentrations should be determined by the researcher: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid freeze-thaw cycles. |
| Storage Buffer | Phosphate-buffered saline, pH 7.4 containing 0.02% sodium azide |
