Application
Western blot analysis of extracts of various cell lines, using MBP antibody.Application
Immunohistochemistry of paraffin-embedded rat kidney using MBP antibody at dilution of 1:200 (400x lens).Application
Immunohistochemistry of paraffin-embedded mouse kidney using MBP antibody at dilution of 1:200 (400x lens).IHC
Immunostaining of the injured spinal cord tissues for choline acetyltransferase (ChAT, green) and myelin basic protein (MBP, green) in SCI, FG, FG-Exo, and Sham groups. Nuclei were stained with DAPI (blue).| Product Name | Myelin basic protein antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myelin basic proteinThe protein encoded by the classic MBP gene is a major constituent of the myelin sheath of oligodendrocytes and Schwann cells in the nervous system. However, MBP-related transcripts are also present in the bone marrow and the immune system. These mRNAs arise from the long MBP gene (otherwise called "Golli-MBP") that contains 3 additional exons located upstream of the classic MBP exons. Alternative splicing from the Golli and the MBP transcription start sites gives rise to 2 sets of MBP-related transcripts and gene products. The Golli mRNAs contain 3 exons unique to Golli-MBP, spliced in-frame to 1 or more MBP exons. They encode hybrid proteins that have N-terminal Golli aa sequence linked to MBP aa sequence. The second family of transcripts contain only MBP exons and produce the well characterized myelin basic proteins. This complex gene structure is conserved among species suggesting that the MBP transcription unit is an integral part of the Golli transcription unit and that this arrangement is important for the function and/or regulation of these genes. [provided by RefSeq, Jul 2008] |
| Immunogen | Recombinant protein of human MBP |
| Isotype | IgG |
|---|---|
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHCIHC-PWB |
| WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | MBP myelin basic protein |
|---|---|
| Target | Myelin basic protein |
| Molecular Weight(MW) | 33 kDa (note) |
Application
Western blot analysis of extracts of various cell lines, using MBP antibody.
Application
Immunohistochemistry of paraffin-embedded rat kidney using MBP antibody at dilution of 1:200 (400x lens).
Application
Immunohistochemistry of paraffin-embedded mouse kidney using MBP antibody at dilution of 1:200 (400x lens).
IHC
Immunostaining of the injured spinal cord tissues for choline acetyltransferase (ChAT, green) and myelin basic protein (MBP, green) in SCI, FG, FG-Exo, and Sham groups. Nuclei were stained with DAPI (blue).| Application Notes | WB 1:500 - 1:2000, IHC 1:50 - 1:200, IF 1:50 - 1:200: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Store at -20℃. Avoid freeze / thaw cycles. |
| Storage Buffer | PBS with 0.02% sodium azide, 50% glycerol, pH7.3. |
