| Product Name | Myeloperoxidase antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal antibody to MyeloperoxidaseMyeloperoxidase is a hemoprotein that is abundantly expressed in neutrophils and secreted during their activation. Native Myeloperoxidase is represented as a covalently bound tetrameric complex of two glycosylated alpha chains (MW 59 - 64 kDa) and two unglycosylated beta chains (MW 14 kDa) with total MW 150 kDa and theoretical pI 9.2. Traditionally Myeloperoxidase was considered as a main target of anti-neutrophil cytoplasm antibodies (ANCA), the serological markers for certain systemic vasculities e.g. periarteriitis nodosa, microscopic polyarteriitis and pulmonary eosinophilic granulomatosis (Churg-Strauss syndrome). Low to moderate anti-Myeloperoxidase autoantibody levels are also reported in rheumatoid arthritis. Recently it was shown that Myeloperoxidase participates in the initiation and progression of cardiovascular disease. It possesses potent proinflammatory properties and may contribute directly to tissue injury. Now Myeloperoxidase is under consideration as one of the most promising cardiac markers. |
| Immunogen | Myeloperoxidase isolated from human granulocytes |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIHC-FIHC-P |
| This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.: |
|
| Species Reactivity | HumanRat |
| Concentration | 0.5mg/ml |
| Purification | Protein A |
| Gene Synonyms | 4353 MPO 104300 P05164 |
|---|---|
| Target | Myeloperoxidase |
| Tissue Specificity | This antibody reacts with human myeloperoxidase. It stains granules of neutrophils granulocytes in spleen, bone marrow, tonsil and blood smears. |
| Cellular Localization | Lysosomal |
| Positive Control | Tonsil or Spleen tissue sections |
|---|---|
| Application Notes | This antibody may be diluted to a titer of 1:25-1:100 in an ABC method. We suggest an incubation period of 30-60 minutes at room temperature. However, depending upon the fixation conditions and the staining system employed, optimal incubation and dilutions should be determined by the user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution, aliquot and store at 4°C. Do not freeze. |
| Storage Buffer | PBS pH7.5 containing 1%BSA and 0.05% sodium azide |
