Application
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD (Phospho-Ser200) Antibody| Product Name | MyoD1 (phospho Ser200) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to MyoD1 (Phospho Ser200)MyoD encodes a nuclear protein that belongs to the basic helix-loop-helix family of transcription factors and themyogenic factors subfamily. It regulates muscle cell differentiation by inducing cell cycle arrest, a prerequisite formyogenic initiation. The protein is also involved in muscle regeneration. It activates its own transcription which may stabilize commitment to myogenesis. |
| Immunogen | Peptide sequence around phosphorylation site of serine 200 (A-S-S(p)-P-R) derived from Human MyoD. |
| Modification | p-Ser200 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | MYF3 MYOD PUM bHLHc1 MYOD1 myoblast determination protein 1 myogenic factor 3 myf-3 class C basic helix-loop-helix protein 1 myogenic differentiation 1 |
|---|---|
| Target | MyoD (Phospho Ser200) |
| Molecular Weight(MW) | 40 (note) |
Application
Western blot analysis of extracts from Hela cells untreated or treated with EGF using MyoD (Phospho-Ser200) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
