Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using myosin IA antibody at 1:500 dilution.Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 5% SDS PAGE diluted at 1:2000| Product Name | Myosin 1A antibody , Internal |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to Myosin 1A (myosin-Ia)The protein encoded by this gene belongs to the myosin superfamily. Myosins are molecular motors that, upon interaction with actin filaments, utilize energy from ATP hydrolysis to generate mechanical force. Each myosin has a conserved N-terminal motor domain that contains both ATP-binding and actin-binding sequences. Following the motor domain is a light-chain-binding 'neck' region containing 1-6 copies of a repeat element, the IQ motif, that serves as a binding site for calmodulin or other members of the EF-hand superfamily of calcium-binding proteins. At the C-terminus, each myosin class has a distinct tail domain that serves in dimerization, membrane binding, protein binding, and/or enzymatic activities and targets each myosin to its particular subcellular location. The kidney epithelial cell line, LLC-PK1-CL4 (CL4), forms a well ordered brush border (BB) on its apical surface. Experiments indicate that the brush border population of the encoded protein turns over rapidly, while its head and tail domains interact transiently with the core actin and plasma membrane, respectively. A rapidly exchanging pool of the protein encoded by this gene envelops an actin core bundle that, by comparison, is static in structure. [provided by RefSeq] |
| Immunogen | Recombinant protein encompassing a sequence within the center region of human Myosin 1A. The exact sequence is proprietary. |
| Clonality | Polyclonal |
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| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | IHC-PWB |
| *Optimal dilutions/concentrations should be determined by the researcher.
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| Species Reactivity | Human |
| Predicted Reactivity | Mouse, Rat, BovineMouse (93%), Rat (94%), Bovine (94%) |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | BBMI antibody DFNA48 antibody MIHC antibody MYHL antibody MYO1A antibody "myosin heavy polypeptide-like (100kD) antibody" brush border myosin I antibody myosin-Ia antibody myosin I heavy chain antibody myosin IA antibody |
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| Molecular Weight(MW) | 118 kDa (note) (2) |
Application
Immunohistochemical analysis of paraffin-embedded human colon carcinoma, using myosin IA antibody at 1:500 dilution.
Application
Sample (30 ug of whole cell lysate) A: Jurkat B: Raji C: K562 D: NCI-H929 5% SDS PAGE diluted at 1:2000| Positive Control | Jurkat , Raji , K562 , NCI-H929 |
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| Application Notes | *Optimal dilutions/concentrations should be determined by the researcher.
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| Form | Liquid |
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| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | 0.1M Tris, 0.1M Glycine, 20% Glycerol (pH7). 0.01% Thimerosal was added as a preservative. |
