Application
HeLa cell lysate spiked with chicken gizzard myosin II left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight| Product Name | Myosin Light chain (phospho Ser19) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to Myosin light chain ( phospho S19 )Myosin light chain (MLC) is a subunit of the conventional myosins (e.g. myosin II). In smooth muscle and non-muscle cells conventional myosins mediate a wide variety of contractile events including cytokinesis, cell motility, and smooth muscle contraction. MLC is phosphorylated by multiple serine-threonine kinases such as Rho-kinase and PAK, however myosin light chain kinase (MLCK) acts as the primary kinase. Contractile activity of conventional myosins is regulated by phosphorylation of MLC on several residues. Phosphorylation of serine 19 (the major phosphorylation site and the preferred site for MLCK) activates myosin motor activity under physiological conditions. |
| Immunogen | Synthetic peptide (Chicken) derived from the region of MLC that contains serine 19 (serine 20 including the initiating methionine). |
| Modification | p-Ser19 |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | WB |
| WB: Use at a concentration of 0.35 - 1.0 μg/ml. Detects a band of approximately 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanChicken |
| Concentration | 1mg/ml |
| Purification | Affinity purified |
| Gene Synonyms | 160780 MYL2 MLC3F 4632 416874 4633 P10916 Myosin Light chain MLC1F CMH10 396470 MYL1 MLC2 160781 P05976 Myosin (phospho S19) Myosin (phospho Ser19) Myosin (pS19) Myosin (pSer19) Myosin phospho S19 Myosin phospho Ser19 Phospho Myosin (S |
|---|---|
| Target | Myosin Light chain |
| Cellular Localization | Cytoplasmic |
Application
HeLa cell lysate spiked with chicken gizzard myosin II left untreated (5) or phosphorylated in vitro by PAK (1-4, 6) were resolved by SDS-PAGE on a 10% polyacrylamide gel and transferred to PVDF. Membranes were blocked with a 5% BSA-TBST buffer overnight| Positive Control | Chicken gizzard myosin II phosphorylated in vitro by PAK |
|---|---|
| Application Notes | WB: Use at a concentration of 0.35 - 1.0 μg/ml. Detects a band of approximately 25 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | Aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. |
| Storage Buffer | Preservative: 0.05% Sodium Azide; Constituents: PBS (without Mg2+ and Ca2+), 1.0mg/ml BSA (IgG, protease free). pH 7.3 |
