| Product Name | MYST antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit polyclonal to MYSTThe MYST family of proteins are transcriptional regulators named after the founding members MOZ, YBF2/SAS 3, SAS 2, and Tip60. MOZ is implicated in acute myeloid leukemia and YBF2/SAS 3 and SAS 2 in transcriptional silencing in yeast, while Tip60, a histone acetyl transferase, is involved in HIV Tat interactions. Additional family members include MOF, which is involved in dosage compensation in Drosphila, and ESA1 a histone acetyltransferase essential for growth in yeast. |
| Immunogen | Synthetic peptide: ENKVGSPEKPLSDLG, corresponding to amino acids 331-345 of yeast ESA1 encompassing a conserved sequence in MYST family members MOZ, YBF2/SAS 3, SAS 2, Tip60, and MOF. |
| Clonality | Polyclonal |
|---|---|
| Isotype | IgG |
| Host Species | Rabbit |
| Tested Applications | ICC/IFIPWB |
| ICC: Use at a dilution of 1/100. IP: Use at an assay dependent dilution. 5ug immunoprecipitates a MYST family member from 1mg of Jurkat RIPA lysate. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
|
| Species Reactivity | HumanMouseZebra FishFish |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Protein A |
| Gene Synonyms | ESA1 MYST 10524 TIP PLIP TIP60 HTATIP Q92993 HTATIP1 601409 cPLA2 |
|---|---|
| Target | MYST |
| Tissue Specificity | Recognizes yeast ESA1 and other mammalian MYST family members. |
| Cellular Localization | Nuclear |
| Positive Control | Jurkat lysate-Use 20ug per lane for minigels - GTX82933 |
|---|---|
| Application Notes | ICC: Use at a dilution of 1/100. IP: Use at an assay dependent dilution. 5ug immunoprecipitates a MYST family member from 1mg of Jurkat RIPA lysate. WB: Use at a concentration of 1 μg/ml. Predicted molecular weight: 59 kDa. Optimal dilutions/concentrations should be determined by the end user.: |
| Form | Liquid |
|---|---|
| Storage Instructions | For long term storage, aliquot and keep at -20°C or below. Avoid repeated freeze-thaw cycles. |
| Storage Buffer | 0.7M Tris-glycine, 0.105M Sodium Chloride, pH 7.4 containing 30% Glycerol and 0.035% sodium azide |
