Application
Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343) Antibody .Application
Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343) Antibody| Product Name | NBS1 (phospho Ser343) antibody |
|---|---|
| Antibody Type | Primary Antibodies |
| Product description | Rabbit Polyclonal antibody to NBS1 (phospho Ser343)Mutations in p95/NBS1 gene are associated with Nijmegen breakage syndrome, an autosomal recessive chromosomal instability syndrome characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. The encoded protein is a member of the MRE11/RAD50 double-strand break repair complex which consists of 5 proteins. This gene product is thought to be involved in DNA double-strand break repair and DNA damage-induced checkpoint activation. |
| Immunogen | Peptide sequence around phosphorylation site of serine 343 (S-L-S(p)-Q-G) derived from Human p95/NBS1. |
| Modification | p-Ser343 |
| Clonality | Polyclonal |
|---|---|
| Host Species | Rabbit |
| Tested Applications | WB |
| Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|
| Species Reactivity | HumanMouseRat |
| Concentration | 1mg/ml (Please refer to the vial label for the specific concentration) |
| Purification | Affinity purified |
| Gene Synonyms | AT-V1 AT-V2 ATV FLJ10155 MGC87362 NBS NBS1 P95 NBN Nijmegen breakage syndrome 1 (nibrin) cell cycle regulatory protein p95 p95 protein of the MRE11/RAD50 complex nibrin |
|---|---|
| Target | NBS1 (phospho Ser343) |
| Molecular Weight(MW) | 95 (note) |
Application
Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343) Antibody .
Application
Western blot analysis of extracts from Hela cells untreated or treated with Hydroxyurea using p95/NBS1 (Phospho-Ser343) Antibody| Application Notes | Suggested starting dilutions are as follows. WB: 1:500~1:1000. Not yet tested in other applications. Optimal working dilutions should be determined experimentally by the end user.: |
|---|
| Form | Liquid |
|---|---|
| Storage Instructions | Keep as concentrated solution. Aliquot and store at -20ºC or below. Avoid multiple freeze-thaw cycles. |
| Storage Buffer | saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. |
